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A rapid and inexpensive PCR-based STR genotyping method for identifying forensic specimens
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A rapid and inexpensive PCR-based STR genotyping method for identifying forensic specimens
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In this study, the Federal Bureau of Investigation's human Combined DNA Indentity System (CODIS) loci were used on a rapid, inexpensive microfluidics chip electrophoresis platform to confirm the identity of forensic samples from an aircraft accident site. Absolute allele identification was not achieved with this method, but it was found to be suitable for comparative analysis, as demonstrated by validation of the results and conclusions from cappllary electrophoresis. The nine CODIS Short Tandem Repeat loci and a gender discrimination locus used in this study have a greater than 5E+07 matching probaility suitable for small sample sizes.
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Title
A rapid and inexpensive PCR-based STR genotyping method for identifying forensic specimens
Call No
TD 4.210:06/14
Digital Link
Click here to access
Online PDF version (FAA-OAM site)
HTML abstract (FAA-OAM site)
Authors
Kupfer, Doris M
United States. Office of Aerospace Medicine
United States. Federal Aviation Administration
Civil Aerospace Medical Institute
Subjects
Forensic genetics.
DNA fingerprinting.
Polymerase chain reaction.
Language
English
Published
Washington, D.C. : Federal Aviation Administration, Office of Aerospace Medicine ; Ft. Belvior, VA : Available to the public through the Defense Technical Information Center ; Springfield, Va. : Available to the public through the National Technical Information Service, 2006.
Publication Desc
i, 14 p. : ill. ;
Series
DOT/FAA/AM 06/14
DOT/FAA/AM (Series) 06/14.
Target Audience
Unknown or not specified
Dimensions
28 cm.